pgam antibody  (GeneTex)

Journal: Oncotarget

Article Title: Insulin/IGF1-PI3K-dependent nucleolar localization of a glycolytic enzyme--phosphoglycerate mutase 2, is necessary for proper structure of nucleolus and RNA synthesis.

doi: 10.18632/oncotarget.4044

Figure Lengend Snippet: Figure 1: Detection of PGAM in KLN-205 cells with antibodies directed to whole PGAM protein or to C-terminal peptide of PGAM. A. control conditions (scan parameters in red channel were set to emphasize nucleolar staining with propidium iodide – PI) B. RNase-treated cells. Bar=15 µm.

Article Snippet: Goat antibodies specific to C-terminal peptide of PGAM (NB100-774) were from Novus Biologicals.

Techniques: Control, Staining

Journal: Oncotarget

Article Title: Insulin/IGF1-PI3K-dependent nucleolar localization of a glycolytic enzyme--phosphoglycerate mutase 2, is necessary for proper structure of nucleolus and RNA synthesis.

doi: 10.18632/oncotarget.4044

Figure Lengend Snippet: Figure 2: Subcellular localization of PGAM with the use of antibody directed to C-terminal peptide of the protein. The localization was examined in cultures of non-small cell lung carcinoma (NSCLC) HL-1 cardiomyocytes, astrocytes and in breast cancer tissue section (BC). Arrows point nucleoli. Bar=5 µm.

Article Snippet: Goat antibodies specific to C-terminal peptide of PGAM (NB100-774) were from Novus Biologicals.

Techniques:

Journal: Oncotarget

Article Title: Insulin/IGF1-PI3K-dependent nucleolar localization of a glycolytic enzyme--phosphoglycerate mutase 2, is necessary for proper structure of nucleolus and RNA synthesis.

doi: 10.18632/oncotarget.4044

Figure Lengend Snippet: Figure 3: Localization of PGAM with the use of antibody against the C-terminal peptide in neoplastically transformed (NSCLC, KLN-205) and non-malignant (HL-1 cardiomiocytes, astrocytes) cells cultured in serum-free media. Bar=10 µm.

Article Snippet: Goat antibodies specific to C-terminal peptide of PGAM (NB100-774) were from Novus Biologicals.

Techniques: Transformation Assay, Cell Culture

Journal: Oncotarget

Article Title: Insulin/IGF1-PI3K-dependent nucleolar localization of a glycolytic enzyme--phosphoglycerate mutase 2, is necessary for proper structure of nucleolus and RNA synthesis.

doi: 10.18632/oncotarget.4044

Figure Lengend Snippet: Figure 4: Localization of PGAM in KLN-205 cells in different culture conditions. PGAM was localized with the use of antibody against the C-terminal peptide of the protein in the cells cultured in serum-free medium re-supplemented with IGF-1, insulin or insulin and inhibitor of PI3K (wortmannin). Bar=15 µm.

Article Snippet: Goat antibodies specific to C-terminal peptide of PGAM (NB100-774) were from Novus Biologicals.

Techniques: Cell Culture

Journal: Oncotarget

Article Title: Insulin/IGF1-PI3K-dependent nucleolar localization of a glycolytic enzyme--phosphoglycerate mutase 2, is necessary for proper structure of nucleolus and RNA synthesis.

doi: 10.18632/oncotarget.4044

Figure Lengend Snippet: Figure 5: Localization of PGAM with antibody directed to the C-terminal peptide of the protein in nuclei of KLN-205 cells treated with drugs disturbing ribosomal biogenesis. ActD – actinomycin D; PI – propidium iodide. Bar=5 µm.

Article Snippet: Goat antibodies specific to C-terminal peptide of PGAM (NB100-774) were from Novus Biologicals.

Techniques: